BIOPROCESSING OF VIRAL

VACCINES

The concept of this book originates from the delivery of the series of Cell Culture-

based Viral Vaccines Course sponsored by the European Society of Animal Cell

Technology (ESACT) https://esact.org/cell-culture-based-viral-vaccines-course/

and its content includes some of the material that has been developed and

adapted over many years.

The book focuses on cell-culture−produced viral vaccines to meet the needs of

the rapidly expanding research and development in academia and industry in the

field. This book introduces the basic principles of vaccination and the

manufacturing of viral vaccines. Bioprocessing of Viral Vaccines will provide an

overview of the advanced strategies needed to respond to the challenges of new and

established viral infection diseases. The first few chapters cover the basics of

virology and immunology as essential concepts to understand the function and

design of viral vaccines. The core of the content is dedicated to process

development, including upstream processing and cell culture of viral vaccines,

downstream processing, and analytical technologies as applied to viral vaccines.

Advanced process analytical technologies (PAT) and quality by design (QbD)

concepts are also introduced in the context of vaccine manufacturing. The case

studies included cover inactivated, attenuated vaccines exemplified by influenza

vaccines; sub-unit vaccines exemplified by virus-like particles (VLPs: HPV

vaccines) and recombinant protein vaccines (Flublock); vectored vaccines:

adenoviruses (AdV) and vesicular stomatitis virus (VSV) vectored vaccines;

genomic vaccines (DNA and mRNA), vaccines as developed for COVID-19

response in particular; and a review of all COVID-19 vaccines approved or in

advanced clinical trials. Primarily this book is aimed at graduate engineers and

professionals in the fields of vaccinology, bioprocessing, and biomanufacturing of

viral vaccines.